Two people lost their lives and 33 others were injured after a Serbian-registered passenger bus overturned on Croatia's A3 motorway on Sunday morning, Croatian authorities said.

The accident occurred at around 7:25 a.m. while the bus was travelling toward Zagreb. Initial findings indicate that the vehicle left its lane, struck a roadside barrier and overturned beside the motorway.

Authorities have launched an investigation to determine the exact cause of the crash.

Major emergency response launched

Croatian health officials said six of the injured were in serious condition, while the remaining 27 sustained minor injuries. Ambulances transported the victims to hospitals in and around Zagreb for medical treatment.

The emergency response involved firefighters, police officers, rescue teams and a medical helicopter, which were dispatched to the scene shortly after the crash was reported.

Officials said the bus was carrying 33 passengers and two drivers when the accident occurred.

Investigation continues into cause of crash

State prosecutors said investigators had completed an initial examination of the scene and were continuing to gather evidence to determine the circumstances that led to the accident.

Authorities also confirmed that the two victims were citizens of Croatia and Montenegro, while forensic examinations have been ordered as part of the investigation.

The crash temporarily disrupted traffic on the A3 motorway, a major transport route connecting the Serbian border with Zagreb and western Croatia, as emergency crews cleared the scene and investigators carried out their work.

Officials said further findings would be released as the investigation progresses.(ILKHA)